Fig. 3. Hemocytes in the clot show hallmarks of apoptosis. Bright field (A) and fluorescence (B) microscopy of a TUNEL assay performed on a clot preparation showing TUNEL-positive (filled arrows) and TUNEL-negative plasmatocytes (open arrows) in the clot. The border of the clot is indicated by arrowheads. (C-E) Phase contrast (C), annexin V (D) and propidium iodide (E) labeling of plasmatocytes 7 minutes after bleeding showing live (^*) as well as apoptotic (open arrow, only annexin V positive) and necrotic cells (solid arrow, reacting with both annexin V and propidium iodide).