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Figure 5


Fig. 5. Srv2 interacts with profilin in vivo. (A) Immunoprecipitation of Srv2 with anti-Srv2 antibody was carried out from wild-type SRV2, srv2-201 and srv2{Delta} yeast strains. The blot was probed with anti-profilin antibody and demonstrates specific co-immunoprecipitation of profilin with wild-type Srv2. By contrast, only very small amounts of profilin were detected in immunoprecipitates from srv2-201 and srv2{Delta} cells. The amounts of profilin in cell lysates before co-immunoprecipitation are shown as a control. (B) Srv2 localizes in patch-like manner in SRV2 and srv2-201 cells. The Srv2 dots occasionally co-localize with cortical actin patches.