Fig. 5. Adhesion markers are not present at sites of microtubule targeting. B16F1 cells were incubated on laminin for 30 minutes, fixed in buffer containing 0.5% glutaraldehyde and 1% Triton X-100, and stained with monoclonal anti-tubulin antibodies and phalloidin. (A) Cells were stained with monoclonal anti-FAK antibodies. Cells were transfected with YFP-paxillin (B) or mRFP-VASP (C). Combined image shows adhesion sites (blue), actin (red) and microtubules (green). Insets (a-c) show combined adhesion site (blue) and actin (red) images of regions indicated by the boxes. Arrowheads indicate position of targeting sites. Graphs show percentage of targeted and nontargeted filopodia bases positive for the adhesion sites markers. Bars, 2 µm. Error bars indicate standard error.