Fig. 7. Brief taxol treatment increases filopodia targeting. (A) B16F1 cells were treated with 3 µM taxol for 30 seconds, 1 minute or 2 minutes, and fixed in buffer containing 1% glutaraldehyde and 1% Triton X-100. Fixed cells were stained with monoclonal anti-tubulin antibodies and phalloidin. Microtubule (green) and actin (red) images are combined. Bars, 2 µm. (B) Time course of the number of peripheral microtubules in cells treated with taxol. The number of peripheral microtubules peaks at 30 seconds and rapidly declines. (C) Percentage filopodia targeted in cells treated with taxol for 30 seconds (^*P<0.01 unpaired t-test). (D) Percentage positive microtubules in cells treated with taxol for 30 seconds. Error bars indicate standard error.