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Figure 1


Fig. 1. Double immunofluorescence staining with anti-synemin (A,C,F,H) and anti-GFAP (B,D,G) of the molecular layer of the dentate gyrus 4 days after entorhinal cortex lesion. Nuclei are stained blue with TOPRO-3. In wild-type mice (A-E), low-magnification images (A,B) show strong synemin and GFAP staining in numerous large cells in the dentate gyrus; at high magnification (C-E) these cells appear to be reactive astrocytes. An overlay image (E) shows colocalization (yellow) of synemin and GFAP. In Gfap-/-Vim-/- mice (F-J), reactive astrocytes were negative for synemin and GFAP (F,G). Some perivascular cells, however, were positive for synemin (H,J); these were also positive for {alpha}-smooth muscle actin (ASMA) (I,J). Bars, 50 µm (A,B,F,G); 25 µm (C-E,H-J).