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Figure 2


Fig. 2. Synemin expression levels in the cerebral hemisphere of 2-day-old wild-type (wt) and Gfap-/-Vim-/- (G-/V-) mice. (A) Western blots show that synemin protein levels are markedly lower in G-/V-than wild-type mice (upper band, {alpha}-synemin; lower band, beta-synemin) and confirm the absence of GFAP and vimentin in G-/V-mice. Actin control blots demonstrate equal protein loading. (B) A 0.8% ethidium-bromide agarose gel loaded with RT-PCR products obtained after amplification with synemin primers shows two bands corresponding to {alpha}- and beta-synemin mRNA in wild-type and G-/V-mice. Lane M shows molecular mass markers, numbers indicate kb. (C) TaqMan real-time PCR amplification curves show no difference in synemin mRNA levels between wild-type mice (circles) and G-/V-mice (triangles). {Delta}Rn, log fluorescence emission intensity of the reporter dye normalized to that of a passive reference dye. Values are mean ± s.e.m. of four different samples. Horizontal line indicates background fluorescence level.