Fig. 8. SW13-cl2 cells transiently transfected with GFAP (A,B) and cotransfected with GFAP and -synemin (C-H). Confocal microscopy revealed three GFAP staining patterns, consisting of a filamentous network with little evidence of bundling (cell on left in A), or a network with some parts displaying large bundles and coils (cell on right in A and E). In overexpressing cells, GFAP formed large rods or comma-like structures (B,G). In cells with little GFAP bundling, synemin did not associate with the GFAP network, but formed dot-shaped accumulations (D). Synemin did associate with large GFAP bundles (F) or overexpression aggregates (H). In cells where only part of the GFAP network formed large bundles, synemin only associated with those parts (compare E with F). Bar, 10 µm.