Fig. 9. SW13-cl2 cells stably transfected with GFAP and transiently transfected with -synemin (A,B) or -synemin (C,D). Double immunofluorescence staining for GFAP (A,C) and synemin (B,D) was performed 48 hours after transfection. Confocal microscopy revealed a filamentous GFAP network in most cells. Neither -nor -synemin associated with this network, instead forming dot-shaped structures not stained by anti-GFAP. Bar, 10 µm.