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Figure 2


Fig. 2. Cyclin B is mislocalised in Hsp90 mutants. (A-D) Immunostaining of Drosophila third instar larvae neuroblasts. Cyclin B is shown in red (left panel), {alpha}-tubulin in green (middle panel), and DNA in blue. (A) In WT prophase cells (A) cyclin B accumulates on centrosomes. (B) In Hsp90 mutant prophase cells cyclin B can be detected on centrosomes (white arrows) but this localisation is weaker and more diffuse than normal. (C) In WT metaphase cells cyclin B stains the centrosomes, the spindle MTs and the spindle mid-zone. (D) In Hsp90 mutant metaphase cells cyclin B strongly stains the spindle mid-zone, but staining on the spindle and centrosomes is much weaker than normal. (E-G) Bar graphs showing the quantification of cyclin B staining on centrosomes or on MTs in WT (green bars) and Hsp90 mutant (red bars) Drosophila neuroblasts (see Materials and Methods) in prophase (E), prometaphase (F) and metaphase (G). Bar, 5 µm (A-D).