Fig. 2. Three-dimensional reconstructions of tubular networks in P. grylli. Cells from cricket fat bodies were infected with P. grylli and fixed chemically (A,C-F) or prepared by high pressure freezing and cryosubstitution (B). 200-nm-thick sections of microsporidia were subjected to EM tomography. (A) General overview of the meront TN surrounded by the ER (green), which contains tubular (brown) and varicose (yellow) parts. Arrow indicates connection between TN and ER. (B-D) TNs in meronts pretreated without (B) and with (C,D) NEM for 15 minutes. (E,F) In the sporont, the varicose part is larger, with the smooth tubular part still connected (E,F, arrows) to the ER (green). (B-D) TNs, red (B) or orange (C,D); ER, green. Bar, 80 nm (A-D); 100 nm (E,F).