Fig. 8. Effect of a dominant negative Rac1 mutant. (A) COS-7 cells were transfected with 1 µg of plasmid expressing the HA-tagged wt Nod2 protein and 2 µg of plasmid encoding the dominant negative Rac1 mutant (Rac N17). Twenty-four hours post transfection, cells were stained for Nod2 and Rac1 with anti-HA and anti-Rac1 mAbs, respectively. Images were obtained by confocal microscopy (Leica TCS NT). (B) COS-7 cells were transfected with 50 ng of (B)₅LUC in the presence or not of plasmid expressing Nod2 (5 ng) or Rac N17 (100 ng). The DNA total amount was adjusted to 500 ng with pcDNA3. Twenty-four hours post transfection, cells were harvested for LUC assays. Values represent the mean + s.d. of triplicate cultures.