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Figure 2


Fig. 2. Immunolocalization of pSMAD2 in preantral (A), transitional preantral-antral (B) and antral follicles before (C) and after (D) 8 hours hCG (5 IU) treatment in vivo. (E) No staining was observed when the primary antibody was pre-incubated with pSMAD2 phosphopeptide. Arrows indicate cumulus-oocyte complexes. Bars, 50 µm. (F) Western blot immunostained for pSMAD2, total SMAD2 and ACTB in freshly isolated COCs and mural cells from antral follicles.