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Figure 4


Fig. 4. Specificity of inhibitors. (A) Western blot of lysates collected from COCs treated with media only, SB431542 (10 µM) or SIS3 (20 µM) for 4 hours and immunostained for pSMAD2, total SMAD2, pSMAD3, pSMAD1/5/8 and ACTB proteins. (B) Western blot immunostained for pSMAD2 and ACTB of COCs treated with increasing concentration of SB431542 (0.1 µM to 100 µM) for 4 hours.