Fig. 7. Regulation of cumulus transcripts (Slc38a3, Amh, and Ar) by oocytes. (A) Effect of OOX and oocyte co-culture on cumulus transcript levels in COCs and OOX cumulus cells cultured in vitro for 12 hours. Effect of SB431542 (10 µM) and FSH (100 ng/ml) (B) or SIS3 (20 µM) (C) on cumulus transcript levels in COCs cultured for 15 hours. Transcripts were measured by real-time PCR and normalized to Rpl19 mRNA. ^abcP<0.05, significant differences.