Fig. 1. Generation of the T-synF allele. (A) Gene targeting scheme. B, BamHI. (B) Southern analysis of BamHI-digested genomic DNA from ES clones after Cre-recombinase-mediated deletion of the Neo selection marker. (C) Southern blot analysis of BamHI-digested genomic DNA from mouse tails identifying T-synF mice. The probe for each Southern analysis was exon 2 of the T-syn gene.