Fig. 4. (A) Immunofluorescence images of actin (a,c,e) and vinculin (b,d,f) in REFs adherent to 10 µg/ml fibronectin (a,b) or PF8 (c,d). In e,f, 1 µg/ml PF10 was added in solution for 30 minutes before fixation. (B) The table shows quantification of the number of cells on FN, PF8, or PF8+PF10 fragments that express vinculin-containing and non-vinculin-containing focal plaques (±s.d.).