Fig. 4. Focal adhesion turnover and disassembly is affected by MT acetylation. (A) Focal adhesion turnover was calculated from FRAP images of WT and HDAC6 KO MEFs expressing paxillin-GFP. Bars, 20 µm. (B) Average half-time (t_1/2) of FRAP of adhesions in HDAC6 WT and HDAC6 KO MEFs expressing GFP-paxillin; n>8 adhesions in each type of MEF. (C,D) Rates of adhesion disassembly (C) and assembly (D) were quantified from the pixel-intensity of individual adhesions in time-lapse images of both HDAC6 WT and HDAC6 KO MEFs that were expressing GFP-paxillin; n>12 adhesions in each type of MEF. ^*, conditions statistically different from control (P<0.05).