Fig. 5. Analysis of filament orientation and fit of simulated optical and EM images to the experimental images. (a) Angular distribution of correlation length in experimental (solid curves) and simulated (dashed curves) images. (b) Comparison of correlation-length distribution in cells treated with 0.1 µM of cytochalasin D with simulated distributions with short (or long) filaments and high (or low) orientational disorder parameters. The angle was measured with respect to the normal to the leading edge. (c-e) Simulated platinum replica images (c-e) created on the basis of the same simulations as in (a) and compared with a real platinum replica image (f) (provided T. M. Svitkina and G. G. Borisy). (g) A simulated negative staining EM image with the same parameters. Diagonal black line is parallel to the leading edge. Bar, 1 µm.