Fig. 1. Schema of the csq-1-null mutant csq-1 (jh109). (A) The 1042 bp deletion, which removed several exons, is shown by a horizontal bar. (1) and (2) indicate primer sets used for nested PCR (indicated by arrows) performed for mutant isolation. (B) Agarose gel of PCR fragments showing bands obtained from single worm PCR. Wild type (+/+), heterozygote (+/–), and homozygote (–/–). Absence of a band in lane labelled (–/–) confirms that the worm is a homozygote. (C) Western blot showing the expression of CSQ-1 and PYP-1 (inorganic pyrophosphatase as control) in wild-type worms (lane 1) and csq-1(jh109) animals (lane 2).