Fig. 2. Phenotypes of wild-type and csq-1 mutant worms. (A) Analysis of motility assays in liquid medium showing the average number of waveforms measured in 1 minute (error bars give s.d., n=20). (B) Analysis of average brood size of the indicated strains (error bars give s.d., n=20). (C,D,E) Survival rate under high-[Ca²⁺] stress conditions, low-[Ca²⁺] stress conditions and DTT stress conditions. Young adult worms were grown on plates containing 20 mM calcium acetate, 20 mM EGTA or 1 mM DTT solution. Surviving worms of each strain were counted after 3 days. The unc-68 gene encodes the RyR and crt-1 encodes calreticulin. csq-1;crt-1 is a double mutant of calsequestrin and calreticulin and csq-1;unc-68 is double mutant of calsequestrin and RyR. (F-H) Electron micrographs of the transverse section showing body-wall muscle structure of wild-type worms (F), csq-1(jh109) mutant worms (G) and csq-1(jh109) mutant worms grown on EGTA plates (H).