Fig. 5. Expression of Myc-RhoB T19N impairs CXCL8-induced CXCR2 degradation following 3 hours of CXCL8 stimulation. Representative western blot analysis of CXCR2 expression in cells transfected with vector or Myc-RhoB T19N in the presence of 20 µg/ml cycloheximide following stimulation with vehicle (Unt) or 100 ng/ml CXCL8 for 30 minutes, 180 minutes or 360 minutes. Actin is shown as a control to monitor for equal loading of protein. Data shown are representative from three separate experiments.