Fig. 7. The UPS reporter system in cortex cultures of Ub^G76V-GFP transgenic mice. (A) Ub^G76V-GFP tg organotypic cortex cultures without treatment with proteasome inhibitors. (B) Ub^G76V-GFP tg cortex cultures treated with 1 µM epoxomicin. The GFP-reporter substrate only accumulated after proteasome inhibition. Bars, 50 µm.