Fig. 8. High Ad-UBB⁺¹ expression causes proteasome inhibition in cortex cultures. High levels of UBB⁺¹ expression with Ad-UBB⁺¹ led to accumulation of UBB⁺¹ without inhibitor treatment. (A) Representative western blot of HEK293 cell lysates transduced with equal MOI of LV-UBB⁺¹ (left lane) or Ad-UBB⁺¹ (right lane). Equal amounts of protein were loaded per lane, as confirmed by Coomassie Blue staining of total protein load of the same lanes shown on the right. The blot was stained with anti-UBB⁺¹ antibody Ub3 and quantified with Imagepro software (quantification not shown). (B,C) Organotypic cortex slice cultures of Ub^G76V-GFP tg mice were transduced with LV-UBB⁺¹, which did not induce UBB⁺¹ accumulation (B) or Ad-UBB⁺¹, which did result in many UBB⁺¹-immunopositive cells (C). (D):UBB⁺¹ accumulation after adenoviral transduction led to accumulation of Ub^G76V-GFP (arrows). Bars, 250 µm (B); 500 µm (C); 50 µm (D).