Fig. 6. Acetylated Cdc8 regulates myosin function in vitro. (A) Bioinformatic analysis reveals divergence within the N-terminus of S. pombe Cdc8. (B) Titration curves for myosin binding to either 50 nM phalloidin-stabilised pyrene-labelled actin alone (light grey line) or actin saturated with unacetylated Cdc8, Cdc8-AS or endogenous Cdc8 (black line). Curves of best fit data are superimposed on each Cdc8 graph (dark grey). The actin curve is present for reference in all graphs. Titration curves for each of the Cdc8 isoforms were sigmoidal in shape, demonstrating that all three have an inhibitory affect on myosin binding, although the inhibition is significantly stronger for the endogenous acetylated Cdc8.