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Figure 7


Fig. 7. TGFbeta3 signaling promotes post-EMT cell migration of MEE cells. (A) Scratch wound assays demonstrated lack of cell migration in unfused or fused MEE cells. Addition of exogenous TGFbeta3 stimulated cell migration of fused cells, whereas addition of dominant-negative LEF1 or Smad4 (DN LEF-1 or DN Smad4, respectively) prevented migration under these conditions. Antisense beta-catenin/{gamma}-catenin oligodeoxynucleotide (AS beta-catenin ODN) did not prevent TGFbeta3-induced migration. Bar, 60 µm. (B) Transwell cell migration assays showed little migration of unfused or fused MEE cells, whereas fused cells exposed to TGFbeta3 were highly migratory. DN Smad4 and DN LEF1 prevented migration, but AS beta-catenin ODN did not (mean ± s.d.; n=3; *P<0.001 compared with Unfused; **P<0.05 compared with + TGFbeta3).