Fig. 7. The P2Y₂R accesses G₁₂ in a complex with v integrins. (A) RGD-dependent association of the P2Y₂R with v integrins and G₁₂. Cells expressing the HA-tagged WT or RGE mutant P2Y₂R and endogenous G_q/11, G₁₂ and v integrins were treated with or without 1 mM UTP for 5 minutes and lysates were subjected to IP with mouse anti-HA antibody conjugated to agarose beads and IB with anti-G₁₂, anti-G_q/11, anti-v integrin, or anti-HA antibody. Cells transfected with the pLXSN vector served as a negative control. (B) G₁₂ associates with v integrins. Human 1321N1 cells expressing cDNA for the WT P2Y₂R and G₁₂ were treated with or without 100 µM UTP for 5 minutes and lysates were subjected to IP with IgG, anti-3 integrin, or anti-v integrin antibody followed by IB with anti-G₁₂, anti-3 integrin or anti-v integrin antibody. Blots representative of three experiments are shown.