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Figure 2


Fig. 2. Ubiquitin and proteasomes accumulate in the nuclei during spermiogenesis. (A) Anti-ubiquitin antibody staining on testes squashes of wild-type flies. Ubiquitin is detectable in young elongating nuclei (arrow) as well as in canoe stage nuclei (arrowhead and double arrow) with the highest expression in early canoe stage nuclei. (B) Expression of the proteasome subunit Pros{alpha}3-eGFP is very strong in young elongating nuclei (arrow), decreases during the canoe stage (arrowhead and double arrow) and is no longer detectable after the canoe stage. (C) Expression of the proteasome subunit Pros{alpha}3T-eGFP (isoform of Pros{alpha}3-eGFP) starts in young elongating nuclei (arrow) and stays in the nucleus during all later stages (arrowhead and double arrow). Bars, 5 µm.