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Figure 2


Fig. 2. PDE4D4 is expressed in PMVECs. (A) Western blot analysis of PDE4 protein expression in caveolin-containing membrane fractions revealed a 115 kDa band similar to the expected molecular mass of PDE4D4 in PMVECs. (B) Schematic diagram depicts the nine known splice variants encoded by the PDE4D gene. (C) Western blot using antibody specific to PDE4D4 identified a 115 kDa protein (center lane) consistent with results using pan PDE4 antibody. Overexpression of PDE4D4 in PMVECs was used as a positive control (right lane). (D) Semi-quantitative RT-PCR confirmed PDE4D4 expression in PMVECs (β actin, loading control).