JCS -- Creighton et al. 121 (1): 110 Figure IG3

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Fig. 3. PDE4D4 interacts with spectrin in pulmonary endothelial cells. (A) Non-erythroid spectrin is comprised of ${alpha}$ II and βII subunits, which form a heterotetramer. PDE4D4 dimers bind ${alpha}$ II subunit SH3 domains. (B) Co-immunoprecipitation studies using ${alpha}$ II spectrin antibody followed by immunoblotting with antibody to PDE4D reveal a 115 kDa band in PMVECs consistent with the PDE4D splice variant PDE4D4. Overexpression of PDE4D4 in PAECs resolved a 115 kDa band that co-immunoprecipitated with ${alpha}$ II spectrin.