Fig. 1. Development of gc-null cells. Wild-type AX3, gc-null and rescued gc-null cells were settled as a monolayer on non-nutrient agar plates and allowed to starve (t=0). Pictures of developing cells were taken during aggregation (t=5 hours) and after completion of formation of fruiting bodies (t=36 hours). To illustrate better the differential behaviour of the cell strains during aggregation, the area in the white box of the first panel is enlarged in the second panel and further enlarged in the third panel. Black bars indicate the scales used for each picture.