Fig. 1. CLUSTAL W alignment of Leishmania major and Trypanosoma brucei putative endoG with mammalian and nematode homologs. Only the partial amino acid sequences encompassing the predicted nuclease domains are shown (arrow marks). Numbers indicate starting and end amino acids used for alignment. The shaded area represents identical residues, and dashes represent gaps. The DRGH-motif, containing the catalytically important amino acid residues, is underlined. The amino acid residues important for activity such as histidine (^*), the cofactor binding asparagine () and glutamic acid (^*) are indicated. The percentage identities of full-length L. major endoG with those of T. brucei, C. elegans (NUCG_CAEEL), human (NUCG_HUMAN) and mouse (NUCG_MOUSE) endoG are shown in the parentheses.