Fig. 6. Effect of overexpression of endoG on differentiation and survival of Leishmania in macrophages. (A) Human macrophages differentiated from monocytes were infected with purified metacyclic parasites from L. major transfectant cultures (KS, EG, EGm) for six hours (10:1 parasite-to-macrophage ratio), stained with Diff-quick reagents, and the numbers of amastigotes in these cultures were determined over a period of 5 days by microscopic observation. The data are expressed as the number of amastigotes per 100 macrophages. Error bars indicate the standard deviation. (B) DAPI and TUNEL staining of infected macrophages at the 6 hour time point. (C) Macrophages infected with either wild-type L. major (WT) or transfectant parasites (KS, EG, EGm) were stimulated with LPS and human IFN-. DAPI and TUNEL stainings after 48 hours of stimulation are shown. DAPI and TUNEL stainings of non-stimulated macrophages infected with wild-type parasites are shown as controls (top panels).