Fig. 8. Effect of knockdown of endoG in T. brucei. (A) Growth of transfected procyclic T. brucei either induced (RNAi+) or non-induced (RNAi–) with tetracycline. Northern blot from induced (+) or non-induced (–) cells collected at day 6 (gray arrowhead) and probed with a [³²P]-labeled T. brucei endoG-specific probe (endoG; inset). Ribosomal RNA bands (rRNA) stained with ethidium bromide and the results of re-probing the blot with a [³²P]-labeled -tubulin probe are shown (inset). (B) EndoG-depleted (RNAi+) cells and un-induced T. brucei controls (RNAi–) were treated with H₂O₂, labeled with TUNEL mix and analyzed by flow cytometry. The area shaded gray indicates the TUNEL-positive cells without H₂O₂ treatment, and the histogram demarcated by a black line overlay indicates TUNEL-positive cells after treatment with 2 mM H₂O₂ for 1 hour. (C) TUNEL-positive cells from RNAi-induced and non-induced controls were quantified by FACS analysis, and the data represented here were obtained from three independent experiments. (^*) indicates significance (P<0.05). Error bars indicate the standard deviation.