Fig. 4. Jel466-immunostaining of young primary spermatocytes of D. melanogaster. Blue, Hoechst 33258 staining; red, Jel466 immunostaining. Young primary spermatocytes were identified according to Cenci et al. (Cenci et al., 1994). All nuclei, prepared according to standard protocols (Piergentili, 2006) show a maximum of four to five small dots that sometimes congregate into large clumps.