Fig. 6. Electrophoretic analysis of high molecular weight (HMW) ploypeptides expressed in D. melanogaster testes. (A) Testis HMW dynein complement from wild-type males reared at either 24±1°C (lane 1), or at the non-permissive temperature of 31±0.5°C (lane 2). The HMW dynein bands named as band 3 and band 5 (Goldstein et al., 1982) are clearly visible only in lane 1. (B) HMW electrophoretic pattern of testis extracts from males reared at 31±0.5°C (lane 1), and extracts of testis dissected from males reared at 24±1°C and immediately transferred at 32±0.5°C for either 2 hours (lane 2) or 1 hour (lane 3); note that lanes 2 and 3 exhibit the same electrophoretic pattern as controls (panel A, lane 1), indicating that bands 3 and 5 are stable under these experimental conditions.