Fig. 2. (A-D) Fluorometer traces showing the effect of CQ on the alkalinisation rate of the DV in (A) CQR 7G8, (B) CQR Dd2-PM2-GFP, (C) CQS D10 and (D) CQS 3D7-PM2-GFP parasites following the addition of concanamycin A. CQ (10 µM) was added to saponin-isolated mature trophozoites 1 minute before the addition of concanamycin A (100 nM; black arrowheads). The data are from single experiments, representative of those obtained in at least three separate experiments for each strain.