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Figure 1


Fig. 1. Co-immunoprecipitation of {alpha}IIbβ3 with SFKs from platelets. (A) Equal amounts of total protein in the Triton-X-100-soluble extract of resting platelets or thrombin-treated (aggregated) platelets were subjected to immunoprecipitation using indicated anti-SFK antibodies. The immunoprecipitates were subjected to immunoblot analysis using anti-β3 antibodies. AP3 antibody (lane 2; anti-β3 antibody; positive control) but not control Ig (lane 1; normal mouse serum) immunoprecipitated β3 integrin (bottom panel). (B) Densitometric analysis (n=3) of blots in panel A. (C) Anti-SFK antibodies immunoprecipitated equal amounts of Src and Fyn from resting and thrombin-treated platelets.