Fig. 5. Identification of the Fyn-binding region within the β3-cytoplasmic domain. (A) Amino acid sequences of β3-deletion mutants used in this study. (B) The different GST-β3-deletion mutants were incubated with the platelet lysates. Proteins were subjected to SDS-PAGE followed by western blot analyses using anti-Fyn antibodies. The protein loading is shown in the Coomassie stain.