Fig. 7. Spreading of platelets on fibrinogen-coated surface. (A) Washed platelets from wild-type (WT) and Fyn^–/– mice were plated onto fibrinogen-coated coverslips. Pooled platelets from three to four mice were used for these studies. Platelet numbers were 10⁶ cells per well in six-well plates. Cells were allowed to adhere for 30 minutes, fixed, stained with fluorescently labeled phalloidin and then images were captured. Scale bar, 10 µm. (B) The cells from six different fields (a total of 200-300 cells) were counted to determine the percentage spread cells. Data from an independent experiment are given in the bottom panel.