Fig. 3. Characterisation of YFP-EML3 localisation. Images show the localisation of the EYFP-EML3 fusion protein in interphase and in the different mitotic phases as indicated. Cells were fixed with methanol at –20°C and stained with antibodies against GFP (middle left panels and red colour in merge),
-tubulin (middle right panels and green colour in merge) and with DAPI to visualise DNA (blue colour in merge). Scale bars: 10 µm.