Fig. 4. Domain analysis of Eml3. Schematic representation is shown at the top of the constructs used to prepare images below. From the full-length (FL) human EML3 (see also Fig. 2), two basic amino acids were replaced to corrupt the nuclear localisation signal (NLS). An N-terminal fragment (amino acids 1-168, N) contained the HELP domain, whereas the N-terminal 168 amino acids including the HELP domain were missing in the N construct. The EYFP-Eml3 fusion constructs (EYFP-Eml3) were visualised in interphase or metaphase, respectively. Cells were fixed with methanol at –20°C and stained with antibodies against GFP (middle left panels and red colour in merge), -tubulin (middle right panels and green colour in merge) and with DAPI to visualise DNA (blue colour in merge). Scale bars: 10 µm.