Fig. 6. Membrane localization of dephospho-β-catenin in the early Xenopus embryo. Cryosections of stage 9 embryos were stained for dephospho-β-catenin (ABC) and total β-catenin (H102) and nuclei were counterstained with DAPI. (A) Selected fields of the ventral presumptive ectoderm and mesoderm (uninjected ventral), dorsal mesoderm (uninjected dorsal), and ventral ectoderm of an embryo injected with 50 pg Wnt8 mRNA (Wnt-injected). In uninjected embryos, ABC stains the outlines of mesodermal cells (me) (arrowheads) but not ectodermal cells (ec) (arrows). Membrane ABC is detected in ectodermal cells of Wnt-injected embryos (arrowheads). Small bright cytoplasmic spots in all three color channels correspond to autofluorescent pigment granules. (B) Higher magnification view of ABC staining of ectodermal (ec) and mesodermal (me) cells of uninjected cells. These two images were obtained by collection of z-stacks followed by 2D nearest neighbors deconvolution and merging 10 images from each stack.