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Figure 6


Fig. 6. PLC{gamma}1 and PLCβ2 isoforms mediate the formation of peripheral protrusions in epithelial cysts. SK-CO15 cells were transfected with either control siRNA or a combination of PLC{gamma}1- and PLCβ2-specific siRNAs, embedded into 3D Matrigel and, 72 hours later, were treated with blebbistatin. Dual PLC{gamma}1/PLCβ2 knockdown caused a dramatic decrease in the level of both PLC isoforms (A) and significantly attenuated the formation of F-actin-rich spikes on the cyst surface (B,C) (*P<0.01).