Fig. 2. Furrow canal compartments are compromised in nulloX embryos. (A,B) Confocal images of cellularizing embryos. (A) Cross-sections showing tear-shaped F-actin furrow canals (green) that lack Dlg staining (red) in wild-type embryos as compared with flattened furrow canals with significant Dlg staining in nulloX embryos. (B) Cross-sections showing tear-shaped Myosin 2 furrow canals (Myo-2, green) that lack Nrt staining (red) in wild-type embryos as compared with flattened furrow canals with significant Nrt staining in nulloX embryos. (C,D) TEM images showing furrows ingressing in cellularizing nulloX embryos. Arrows indicate the position of furrow canal, shown at higher magnification in the right-hand images. (C) Furrow canal broadenings are often collapsed at the furrow tip (arrowheads in the higher-magnification image). (D) Alternatively, furrow canals may contain PM blebs and show accentuated basal flattening (arrowheads). Scale bars: 5 µm in A,B and 2 µm in C,D.