Fig. 5. Furrow canal compartments are established and maintained in anillin-deficient embryos. (A-E) Confocal images of cellularizing embryos derived from anillinHP/RS or wild-type mothers. (A) En face images from a single plane, showing that Septin (green) fails to accumulate in furrow canals [Myosin 2 (Myo-2), red] of anillin-deficient mutants. (B) Cross-section of an anillin-deficient mutant showing that furrow canals (Myo-2, green) lack staining for a lateral PM probe (Dlg, red). (C) Cross-section of an anillin-deficient mutant showing that collapsed furrow canals (Myo-2, green) lack staining for a lateral PM probe (Nrt, red). (D) En face images from a single plane, showing that Nullo (green) accumulates normally in furrow canals of anillin-deficient mutants. (E) Cross-sections showing F-actin (phalloidin) in cellularizing embryos. Images collected at the same settings show no difference between F-actin levels for wild-type versus anillin-deficient embryos. (F) F-actin levels quantified in furrow canals of wild-type (black) versus anillin-deficient (red) embryos at progressive phases of cellularization, as measured by fluorescent intensity of phalloidin staining. Each point represents one embryo in which 75-100 furrow canals were analyzed. Error bars represent s.d. Scale bars: 5 µm.