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Figure 4


Fig. 4. Securin binds to βTrCP in vivo. (A) Anti-securin and preimmune (PI) sera were used in immunoprecipitations from NP40 extracts of COS-7 cells transiently transfected with pCS2-HA-βTrCP and treated with LLnL for 4 hours before harvesting. The immunoprecipitates were probed for the presence of HA-βTrCP and securin. Lysate, lysate from LLnL-treated COS-7 pCS2-HA-βTrCP cells. (B) COS-7 cells transiently transfected with pCS2-HA-βTrCP{Delta}F were left untreated (Control) or irradiated (UV) before harvesting. Extracts were used for coimmunoprecipitation experiments. Lysate, lysate from COS-7 pCS2-HA-βTrCP{Delta}F cells. Asterisk indicates IgG light chains.