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Figure 3


Fig. 3. Deletion of amino acids 6-28 interrupts AAM-B targeting to droplets. The indicated AAM-B mutants were expressed in HeLa cells and processed for immunofluorescence. (A-C) Deletion of amino acids 2-18 (N2) did not disrupt the targeting of AAM-B (A) to Bodipy 493/503-positive (B) lipid droplets (C). (D-F) AAM-B lacking amino acids 6-28 (N3) had a cytosolic distribution (D). Brightly staining regions (arrows) appear to be insoluble aggregates that do not overlap with Bodipy 493/503-stained (E) lipid droplets (F). (G-I) AAM-B with amino acids 1-23 of CYP2C9 substituted for amino acids 1-28 ({delta}N) had a reticular pattern (G) that was not even weakly associated with Bodipy-positive (H) droplets (I). Scale bars: 5 µm.