Fig. 4. BDNF-enhanced hippocampal dendrite outgrowth is blocked by Src inhibitors but not by the Crk&CrkL shRNA virus. BDNF enhances the growth of Map2-positive hippocampal dendrites 1.8-fold after 5 days of treatment, as compared with growth in neurobasal/B-27 media alone. Inclusion of the SFK family kinase inhibitors PP1 and PP2 during incubation prevented the BDNF-induced augmentation of process extension. However, reduction of Crk and CrkL expression did not significantly block the effect of BDNF on dendrites. The number of neurons measured is indicated at the base of each bar. ^*P<0.001. Error bars indicate s.e.m.