Fig. 2. (A-D) FISH with human chromosome-2-specific pools of large-insert clones representing early-replicating (green) and late-replicating (red) loci to (A) human (schematic of human chromosome 2, together with the mapping position of large-insert clones is shown alongside) and (B) gorilla metaphase chromosomes. (C,D) 3D maximum intensity projections of hybridized human fibroblast and gorilla lymphoblastoid cell nuclei, with early clones labeled green, late clones red, chromosome 2 territories blue and DAPI counterstain gray. Scale bar: 5 µm. (E-J) Quantitative evaluation of radial probe distributions using 3D-RRD or eADS software (n=number of nuclei). The distribution of chromosome 2 clone pools (E) in human fibroblasts, (F) in human lymphoblastoid cell nuclei and (G) in gorilla lymphoblastoid cell nuclei. (H,I) The radial probe distribution with respect to the human chromosome 2 territory and (J) the localization of individual clones 2-3l and 2-6l and the chromosome 2 territory in human fibroblast nuclei.