Fig. 4. p53^+/R246S ES cells are as highly tumorigenic as the p53^–/– cells. (A) Representative images of livers from scid mice injected with ES cells of various p53 genotypes. Four mice per group were used, all of which gave consistent results. (B) Fluorescence microscopy of ES-cell-injected livers before fixation. Green fluorescence indicates the expression of GFP in the tumour nodules in livers. (C) Histological analysis of livers injected with ES cells of various p53 genotypes. The tumour portions derived from the differentiated ES cells are highlighted by dotted lines. Normal liver is indicated by `Li', whereas `B', `C', `E', `F' and `M' indicate the bone, cartilage, epithelium, fibroblast-like cells and smooth muscles, respectively.